Sample

Adult participants gave written informed consent and minors gave written informed assent with consent from a parent or guardian. Participants were included if they were treatment-seeking out-patients of 13–40 years of age who met diagnostic criteria for a possible prodromal syndrome. People were excluded for any of the following reasons: (a) past or current DSM–IV criteria (American Psychiatric Association, 1994) for any lifetime psychotic disorder; (b) they were judged clinically to have a psychiatric disorder (e.g. mania, depression, attention-deficit hyperactivity disorder) which could account for the symptoms; (c) they presented with symptoms occurring primarily as sequelae to drug or alcohol use; (d) alcohol or drug misuse or dependence in the past 3 months; (e) use of antipsychotic medication in the previous 3 months; (f) change in dosage of any antidepressant within 6 weeks, stimulant medication within 4 weeks, or mood stabiliser within 4 weeks.

The Criteria of Prodromal Syndromes (COPS; Reference Woods, Miller and McGlashanWoods et al, 2001) were used to identify those possibly prodromal. The COPS are based on sub-threshold levels of positive symptoms and operationally define three prodromal syndromes (Reference Yung, Phillips and McGorryYung et al, 1998): attenuated positive symptom syndrome, brief intermittent psychotic syndrome, and genetic risk and recent functional decline syndrome. The COPS and the three syndromes are described in detail elsewhere (Reference Woods, Miller and McGlashanWoods et al, 2001; Reference Miller, McGlashan and RosenMiller et al, 2003a ). Individuals were assessed to determine whether the COPS were met by using the Structured Interview for Prodromal Syndromes (SIPS; Reference Miller, McGlashan and WoodsMiller et al, 1999). Reliability of the COPS diagnosis of possible prodrome has been excellent when using the SIPS (Miller et al, Reference Miller, McGlashan and Rosen2002, Reference Miller, McGlashan and Rosen2003a ), and patients thus diagnosed are symptomatic (Reference Miller, Zipursky and PerkinsMiller et al, 2003b ), functionally impaired (Reference Miller, Zipursky and PerkinsMiller et al, 2003b ), cognitively impaired (Reference Hawkins, Addington and KeefeHawkins et al, 2004a ) and treatment-seeking (Reference Preda, Miller and RosenPreda et al, 2002).

Study design

Participants were enrolled between October 2004 and February 2006. The Yale Human Investigation Committee Institutional Review Board approved the protocol. The trial is registered with ClinicalTrials.gov (NCT00237874). This was an open-label study at one site for 8 weeks, followed by an open-label extension phase with monthly follow-up visits to 52 weeks. Findings from the extension phase will be reported subsequently.

Procedure

During the 1–2 weeks prior to beginning study medication, participants underwent eligibility and neuropsychological examinations. After beginning study medication, participants were scheduled for eight weekly visits.

Dosing followed a fixed-flexible schedule. Initial doses were 5 mg/day aripiprazole; after 1 week, the dose was scheduled for increase to 10 mg/day and after 2 weeks to 15 mg/day, unless adverse effects dictated a slower titration schedule. After the third week, the dose could be increased further to 20 mg/day and if needed to 30 mg/day should the person not be responding. Aripiprazole was prescribed as a single daily dose unless there was a reason to divide the dose. The number of milligrams prescribed and the number taken were recorded each day; these data were used to calculate the percentage adherence since the previous visit. Drowsiness was managed initially by switching the timing of the daily dose to bedtime or by dividing the dose. Insomnia was managed initially by switching the timing of the daily dose to early morning. Lorazepam was used to treat insomnia or agitation. Lorazepam or the anticholinergic benztropine was permitted for extrapyramidal symptoms (EPS). Participants continued doses of antidepressant, mood stabiliser, or stimulant medication prescribed before consent but were not permitted to begin or increase dosage of these medications after consent. Individual and family psychosocial interventions with supportive and psychoeducational components were available to each participant.

Assessments

The primary efficacy measure for the analysis of acute treatment was change over time in the total score of the Scale of Prodromal Symptoms (SOPS; Reference Miller, McGlashan and WoodsMiller et al, 1999), a 19-item scale with items scored 0–6. The interrater reliability has been excellent (Reference Miller, McGlashan and RosenMiller et al, 2003a ). Factor analysis supports the validity of the SOPS sub-scales (Reference Hawkins, Quinlan and MillerHawkins et al, 2004b ). Treatment response was defined as all five SOPS positive symptom items being rated below the prodromal range (i.e. ≤2).

Secondary efficacy assessments included the Calgary Depression Scale for Schizophrenia (CDSS; Reference Addington, Addington and SchisselAddington et al, 1990), the Young Mania Rating Scale (YMRS; Reference Young, Biggs and ZieglerYoung et al, 1978), the Beck Anxiety Inventory (BAI; Reference Beck, Epstein and BrownBeck et al, 1988), the Global Assessment of Functioning Scale (GAF; Reference HallHall, 1995), the Heinrichs–Carpenter Quality of Life role functioning sub-scale (HCRF; Reference Heinrichs, Hanlon and CarpenterHeinrichs et al, 1984), and the Social Functioning Scale (SFS; Reference Birchwood, Smith and CochraneBirchwood et al, 1990).

Neuropsychological assessments included tests of attention and working memory: the Continuous Performance Task (CPT; Reference Cornblatt, Risch and FarisCornblatt et al, 1988), identical pairs version, letter number sequencing, N-back, Trails A and B; Stroop Color Word Test for processing speed; Auditory Verbal Learning Task (AVLT; Reference ReyRey, 1964), using alternate forms for verbal memory; and the Wisconsin Card Sort Test (WCST; Reference Heaton, Chelune and TalleyHeaton et al, 1993), semantic (category) fluency, and Controlled Oral Word Association (FAS; Reference Spreen and BentonSpreen & Benton, 1969) test of phonemic fluency for executive functioning.

Abnormal involuntary movements and EPS were assessed by observation and administration of the Simpson–Angus Scale (SAS; Reference Simpson and AngusSimpson & Angus, 1970), the Barnes Akathisia Scale (BAS; Reference BarnesBarnes, 1989), and the Abnormal Involuntary Movement Scale (AIMS; Reference BranchBranch, 1975). Safety was also assessed by analysing treatment-emergent adverse events (Systematic Assessment For Treatment-Emergent Events, SAFTEE, specific inquiry method; Reference Levine and SchoolerLevine & Schooler, 1986), vital signs and weight. Treatment-emergent adverse events were defined as those first occurring or worsening after baseline. All of the above measures were assessed at baseline and weekly thereafter.

Statistical analyses

For the present report, the time frame was the first 8 weeks after beginning study medication, and the principal outcome measure was the SOPS total score. Analyses were based on the intent-to-treat (ITT) principle. All participants were included in the analysis. The principal outcome measure was addressed using a mixed-effects likelihood-based repeated measures linear model (MMRM, as implemented in SAS PROC MIXED) on post-baseline change scores, using baseline scores as a covariate. For other measures we used t-test end-point models at 8 weeks, carrying forward the last observation (LOCF).

Participants

A total of 15 participants were enrolled. Demographic and treatment characteristics at baseline are shown in Table 1. All were diagnosed with the common attenuated positive symptom syndrome putative prodrome subtype according to the COPS and none also qualified for either of the other two less common COPS prodromal syndromes. All but two had never received antipsychotic medication prior to participating. One participantt had received antipsychotic for 5 weeks 5 years earlier, and the other for 6 months ending 17 months before baseline, both for indications other than psychosis. Scores for severity of illness at baseline are shown in Table 2. Although there are a dearth of age-specific normative data (our sample with test–retest data has a mean age of 15.5 years, s.d.=1.3), baseline data (Table 3) suggest mild neuropsychological impairment similar to that observed in our previous prodromal sample (Reference Hawkins, Addington and KeefeHawkins et al, 2004a ). A higher mean AVLT total score was reported for a younger sample by Spreen & Strauss (Reference Spreen and Strauss1998) and substantially better Trail-Making Part A and Part B performances were reported for a healthy sample aged 15–17 years by Fromm-Auch & Yeudall (Reference Fromm-Auch and Yeudall1983).

Table 1 Demographic and treatment data at baseline

Characteristic
Age, years: mean (s.d.)	17.1 (5.5)
Male, n (%)	8 (53)
White, n (%)	11 (73)
Single, n (%)	15 (100)
First-degree family history, n (%)
Psychosis	3 (20)
Non-psychotic major depression	6 (40)
Non-psychotic bipolar disorder	1 (7)
Medication use at baseline1, n (%)
Antidepressants	7 (47)
Antipsychotics	0 (0)
Benzodiazepines/hypnotics	2 (13)
Mood stabilisers	0 (0)
Stimulants	1 (7)
Lifetime substance
misuse/dependence, n (%)
Marijuana	1 (7)
Other (except nicotine)2	0 (0)

Table 2 Baseline and change from baseline in severity of illness scores of participants treated with aripiprazole

	Mean
Measure	Baseline	Change from baseline1	t (14)2, P
SOPS Total	43.3 (15.0)	-29.1 (12.3)	-9.2 < 0.001
Positive	13.6 (3.7)	-10.0 (4.1)	-9.4 < 0.001
Negative	12.9 (6.9)	-8.2 (6.5)	-4.9 < 0.001
Disorganisation	7.1 (3.8)	-4.8 (3.3)	-5.6 < 0.001
General	9.7 (4.2)	-6.1 (2.6)	-9.1 < 0.001
YMRS total	4.7 (3.7)	-3.9 (3.6)	-4.3 < 0.001
CDSS total	4.6 (4.5)	-3.8 (4.2)	-3.4 0.005
BAI total	14.9 (12.3)	-13.1 (11.2)	-4.5 < 0.001
Current GAF	41.5 (6.4)	9.2 (5.3)	6.7 < 0.001
HCRF	14.2 (4.7)	3.2 (5.1)	2.3 0.044
SFS	118 (30)	-2 (21)	-0.3 0.734

Table 3 Baseline and change from baseline in neuropsychological functioning measures (n=10)¹

	Baseline1	Change from baseline1,2
Domain	Mean (s.d.)		t (9)1 P
Attention and working memory
CPT—IP D-prime (2 digit)	3.5 (0.7)	-0.3 (0.4)	-2.5 0.040
CPT—IP D-prime (3 digit)	2.3 (0.7)	-0.2 (0.8)	-0.5 0.598
CPT—IP D-prime (4 digit)	1.2 (0.5)	-0.2 (0.6)	-0.8 0.452
CPT—IP reaction time (2 digit)3	454 (39)	31 (40)	2.2 0.066
CPT—IP reaction time (3 digit)3	477 (79)	27 (60)	1.3 0.249
CPT—IP reaction time (4 digit)3	469 (48)	-14 (64)	-0.6 0.547
Letter number sequencing	12.9 (4.3)	-0.3 (1.7)	-0.6 0.563
1-back number correct	47.5 (17.9)	-0.8 (15.7)	-0.1 0.896
2-back number correct	26.8 (16.1)	9.1 (10.5)	2.5 0.044
Trails A3	31.9 (11.3)	0.3 (7.0)	0.1 0.890
Trails B3	95.9 (54.2)	2.3 (37.9)	0.2 0.858
Processing speed
Stroop colours	59.9 (15.2)	-0.8 (11.4)	-0.2 0.829
Stroop words	82.0 (21.0)	-2.1 (14.1)	-0.5 0.648
Stroop colour-word	35.5 (8.5)	0.0 (3.5)	0.0 1.000
Verbal memory
AVLT immediate trials sum	47.7 (11.7)	0.4 (13.5)	0.1 0.927
AVLT delay trial	10.5 (2.6)	-0.3 (2.4)	-0.4 0.703
Executive functioning
WCST perseverative errors3	10.9 (6.4)	3.6 (5.2)	2.1 0.072
WCST categories completed	4.0 (0.9)	0.0 (0.7)	0.0 1.000
Semantic fluency	36.9 (10.1)	-4.3 (4.0)	-3.4 0.008
Phonemic fluency	30.6 (8.9)	-1.1 (5.1)	-0.7 0.513

Thirteen participants completed the 8-week study (87%). Of the two drop-outs, one completed 48 days on aripiprazole and dropped out because of improvement, feeling medication was no longer needed. The other completed only 8 days on medication and left the study primarily because of sedation after the first 10 mg dose, after having concluded that 5 mg was ineffective after the first week.

Medication

Prescribed mean (s.d.) aripiprazole doses at weeks 1, 2, 3, 4, 5, 6, 7 and 8 were 5 (0), 9 (2), 11 (5), 11 (7), 11 (6), 13 (6), 14 (8), and 15 (7) mg/day, respectively. Final prescribed doses were 5 mg/day (n=1 participant), 10 mg/day (n=3), 15 mg/day (s.d.=7) (n=6), 20 mg/day (n=4) and 30 mg/day (n=1). Reported mean (s.d.) percentage adherence with prescribed aripiprazole doses was 94 (13), 92 (22), 98 (4), 96 (8), 97 (7), 95 (10), 98 (5), and 94 (9) at weeks 1, 2, 3, 4, 5, 6, 7 and 8 respectively. New concomitant medication used after enrollment included benzodiazepine in three participants (20%) and anticholinergic medication in 5 participants (33%).

Fig. 1 Change in Scale of Prodromal Symptoms (SOPS) total score rom baseline with aripiprazole treatment. Mixed-effect repeated-measures model-derived mean and standard error are shown for each time point. *P<0.05, **P<0.001.

Efficacy

In the mixed-effects model, the effect of time for the SOPS total score change from baseline was statistically significant (F (7)=9.2, P<0.001. The reduction in the SOPS total score was statistically significant at each time point (Fig. 1). The LOCF analyses revealed that improvement on the SOPS was statistically significant at end-point for each of the positive, negative, disorganisation and general symptom subscales (Table 2). The LOCF analyses also revealed significant symptomatic improvement from baseline on the YMRS, CDSS and BAI scales, as well as significant functional improvement on the GAF and HCRF scales (Table 2).

A total of 11 participants met response criteria (73%) at week 2 (n=1), 3 (n=2), 4 (n=1), 6 (n=4), 7 (n=1) and 8 (n=2). Response was sustained thereafter until end-point in all but one participant. No participant converted to psychosis. Two non-responders who completed the 8-week course elected not to continue aripiprazole after 8 weeks; the remaining 11 who completed 8 weeks elected to continue on aripiprazole into the extension phase.

Table 3 shows the results of the neuropsychological testing. Participants improved as a group on two tests of attention and working memory at the significant or trend level (2-digit CPT reaction time, 2-back number correct) but worsened on one other (2-digit CPT performance). They improved as a group on a test of executive functioning (WCST perseverative errors) but worsened on another (semantic fluency). Scores on the remaining 15 tests of attention, working memory, executive functioning, processing speed and verbal memory did not change significantly.

Safety

One participant discontinued aripiprazole because of adverse events (sedation after 8 days). As determined by the SAFTEE, there were few adverse events of more than mild severity (Table 4). Complaints of adverse events tended to remit over time. Other than the participant who took medication for only 8 days, at the final evaluation an emergent SAFTEE complaint of moderate or greater severity was present in only one participant (nasal congestion).

Table 4 Treatment-emergent adverse events in participants with prodromal symptoms treated with aripiprazole

SAFTEE items1	n (%)
Irritability	5 (33)
Increased appetite	4 (27)
Sedation	3 (20)
Insomnia	2 (13)
Nervousness	2 (13)
Memory impaired	2 (13)
Sensory perception impaired	2 (13)
Saliva increased	2 (13)
Libido decreased	2 (13)
Excessive sweating	2 (13)

During treatment, eight participants experienced emergent akathisia demonstrated by increases from baseline on the BAS. Emergent akathisia was managed by slowing dose titration (1), prescribing anticholinergic medication (2), slowing dose titration and prescribing anticholinergic medication (2), slowing dose titration and prescribing benzodiazepine (2) and prescribing anticholinergic medication and then adding benzodiazepine (1). In the context of these management efforts, all participants experiencing emergent akathisia completed treatment, and the emergent akathisia remitted by the final evaluation in six participants. Mean BAS total scores consequently returned to baseline by the end-point evaluation (Table 5). Four participants continued to receive medication for akathisia at the 8-week evaluation.

Table 5 Baseline and change from baseline in vital signs, weight, extrapyramidal symptoms and abnormal involuntary movements

	Baseline	Change from baseline1
Measure	Mean (s.d.)		t (14)2 P
Blood pressure, mmHg
Systolic	120 (12)	3 (12)	1.0 0.338
Diastolic BP	65 (10)	1 (9)	0.5 0.635
Pulse, beats/min	69 (11)	6 (14)	1.4 0.181
Weight, kg	67.4 (18.1)	1.2 (2.0)	2.2 0.049
SAS total score	0.6 (0.9)	-0.3 (0.8)	-1.5 0.136
BAS total score	1.2 (1.3)	-0.7 (1.6)	-1.6 0.136
AIMS total score	0.1 (0.5)	-0.1 (0.5)	-1.0 0.335

Little change from baseline to end-point was observed for blood pressure (Table 5). Pulse increased 6 beats per minute on average. There were no significant differences from baseline to end-point on the SAS or AIMS scales (Table 5). Patients gained a mean of 1.2 kg in weight (Table 5).

Other studies

No participants converted to psychosis during the 8-week trial. We would have expected two or three conversions without treatment, based on the placebo group in our previous study (Reference McGlashan, Zipursky and PerkinsMcGlashan et al, 2006). Caution is indicated in comparing our current findings with our historical placebo group, however, because it is possible that people volunteering for an open-label trial could differ from those volunteering for a placebo-controlled study (Reference Woods, Gueorguieva and BakerWoods et al, 2005).

This is the second report to our knowledge to focus on an acute pharmacological treatment of symptoms that can be prodromal for schizophrenia. Our findings can be compared with those from the acute phase of our previous placebo-controlled trial of olanzapine (Reference Woods, Breier and ZipurskyWoods et al, 2003), but again one must allow for the different study designs. Participants with established schizophrenia improved less from baseline to end-point on active medication when placebo-controlled designs were used (Reference Woods, Gueorguieva and BakerWoods et al, 2005). It is not known whether this same effect of design occurs with putatively prodromal subjects. However, improvement in prodromal symptoms and treatment completion rates in participants assigned to aripiprazole compared favorably with these indices in participants randomised to olanzapine in our previous study. Participants were slightly more severely ill at baseline in the current sample according to the SOPS total score, although GAF scores at baseline and sample demography were similar. Improvement on GAF, as well as on mania and depression measures, was also more robust in the open-label study of aripiprazole than we had previously observed in a marked study of olanzapine.

Weight gain with aripiprazole also compared favourably with our previous experience with olanzapine. Participants treated with olanzapine gained a mean of 4.5 kg over 8 weeks in LOCF analyses, whereas the aripiprazole mean weight gain in this study was 1.2 kg (Table 5) despite being exposed to drug for a higher proportion of the 8 weeks. This degree of weight gain was comparable to that observed in previous short-term studies of aripiprazole (mean 0.71 kg v. no change for placebo; Reference Marder, McQuade and StockMarder et al, 2003).

Akathisia, on the other hand, was more problematic with aripiprazole than it had been with olanzapine, leading to higher rates of benztropine prescription. However, benztropine, or in some cases slowing of dose titration or benzodiazepine prescription, was effective in managing this adverse effect, so that by the final evaluation the net effect of treatment on akathisia ratings was similar to what we had observed with olanzapine.

Adherence with prescribed doses was relatively high in this short-term analysis as measured by participant report at each visit. Adherence was similar to or higher than with olanzapine in our masked study, although the latter used a somewhat more rigorous pill-count method of monitoring adherence (Reference Woods, Breier and ZipurskyWoods et al, 2003). As for most studies, surreptitious non-adherence cannot be excluded. Future studies should continue to assess adherence in this population.

Our neuropsychological findings show little consistent effect of aripiprazole over 8 weeks. Among the 20 results reported, a few tests did show improvement, but others showed a decline, with most suggesting little change. Considering tests that did change, within domain of function there appeared to be little consistency in direction. Certainly the limited sample size would have predisposed our study to low power to detect possible real effects; on the other hand, some gains would be expected on the basis of prior test exposure. In the only previous study, to our knowledge, on neuropsychological effects of aripiprazole (Reference Kern, Green and CornblattKern et al, 2006), 169 participants with chronic schizophrenic psychosis underwent neuropsychological examinations before and 8 weeks after random assignment to aripiprazole or olanzapine. Aripiprazole-treated participants improved significantly over 8 weeks on a general cognitive factor on which loaded our letter number sequencing, verbal fluency and trail-making tasks. Our failure to find consistent improvement on these measures could relate to our small sample or to our participants being less impaired and having less room to improve. Aripiprazole-treated participants improved significantly in the previous study (Reference Kern, Green and CornblattKern et al, 2006) on a verbal learning factor on which loaded a test similar to our AVLT but without use of alternative forms. Our failure to find consistent improvement on verbal memory could relate to the small sample, studying prodromal v. chronic illness, or, our use of a counterbalanced alternate form for the repeat verbal learning list, which may have prevented the confounding of measurement of new verbal learning by familiarity with the word list carried over from the baseline testing (practice effect; Reference Hawkins and WexlerHawkins & Wexler, 1999; Reference Hawkins, Dean and PearlsonHawkins et al, 2004c ). In the previous study (Reference Kern, Green and CornblattKern et al, 2006) aripiprazole-treated participants improved but not significantly on an executive functioning factor on which loaded our WCST tasks. These findings were similar to ours with WCST perseverative errors.

Dosing

We paid close attention to the dosing of aripiprazole in the current study. Although some investigators have emphasised that patients meeting prodrome criteria can be managed with antipsychotic doses which are lower than those used in chronic schizophrenia (Reference FalloonFalloon, 1992; Reference McGorry, Yung and PhillipsMcGorry et al, 2002), there had been some evidence in our previous study that olanzapine had been used at too low a dose, especially in the first month (Reference McGlashan, Zipursky and PerkinsMcGlashan et al, 2006). In the present study we employed a recommended fixed dose titration by the end of the second week to 15 mg/day, the minimal consistently effective aripiprazole dose for patients with chronic schizophrenia (Reference WoodsWoods, 2003), unless the prescriber had a specific reason to deviate from this schedule. The 15 mg/day dose was also that most commonly employed in a recent pilot study of aripiprazole in first-episode schizophrenia (Reference Brown, McEvoy and SahaBrown et al, 2003). Initial doses were 5 mg/day, in keeping with guidelines for initiation of aripiprazole in adolescents/children who weigh 50–70 kg (Reference Findling, Blumer and KauffmanFindling et al, 2004).

Implications

Our current findings are relevant to discussions of the ethics of intervention research in people who appear prodromal. Prodrome research studies thus far have primarily focused on preventing the development of psychosis. Although this is certainly an important goal, ethical issues are raised because some participants will be false-positives who have no personal opportunity to benefit if benefit is defined solely as prevention. The current data suggest that people carrying a risk of progression to psychosis can receive not only the possibility of a preventive benefit but also a treatment benefit ‘on average’ from intervention. The prospect of treatment benefit on average is generally considered sufficient to justify exposure to some treatment risk in other illnesses. Thus the current data strengthen the argument that intervention studies can be ethical with people who appear prodromal.

Although our findings suggest that people who meet prodrome criteria benefit when prescribed aripiprazole, the present results contribute to what is only the beginning of the process of establishing a standard of care for such people. The sample size in our study was small and we had no control group, placebo or otherwise. Future placebo-controlled studies with more participants are needed before recommendations can responsibly be made regarding routine treatment. The present findings suggest that aripiprazole is a promising candidate for such studies.